NDV as an Oncolytic Agent - Study in Cancer Cell Lines

Newcastle disease virus (NDV) exhibits oncolysis in its natural form. This oncolytic (OV) has the potential to specifically infect, propagate, and lyse cancer cells while sparing normal cells. study was aimed screen for NDV strain isolated from poultry. A total of ten velogenic strains were propagated 10 day old embryonated SPF chicken eggs allantoic fluid these infected collected further study. The enumeration carried out by hemagglutination assay (HA) end point dilution method. cytopathic effect on cell lines like MDA-MB-231, MCF-7, PC3, A549 along with control line HEK293 determined MTT 72 hours post infection. These three doses (1, 0.1, 0.01 MOI). DNA laddering screened isolate studied after infecting all at MOI 1. Morphological changes MDA-MB-231 infection analyzed using H&E hematoxylin eosin staining. showed maximum i.e. 61.55% 1 but had no potent cytotoxic HEK293. observed which confirmed mode death be apoptosis. All morphological typical cytopathogenic effects lines. In conclusion, shows effective against line. However, is required determine exact action involved.